The calls are getting louder. Even Russia, Syria's main ally, is now urging the Syrian government to allow weapons inspectors to investigate the site of Wednesday's alleged chemical attack on the suburbs of Damascus, held by rebels opposing President Bashar al-Assad's regime. This brings Russia in line with the US and the 36 other countries that yesterday called for a swift investigation into the apparent deaths of over 1000 men, women and children.
Even if the inspectors – who are already in the country looking into three other unverified attacks – are given permission to investigate the site, there is no guarantee that they will be able to establish conclusively what has happened. But the tension is cranking up. US president, Barack Obama, who a year ago described the use of chemical weapons as a "red line", today said that if the claims were verified, the situation "would require America's attention".
Right now, the evidence that a chemical attack has taken place is based on video footage of the people affected, eye-witness accounts and images of what appears to be the remnants of a delivery rocket. All point to a chemical weapon attack, but there is the possibility, albeit an unlikely one given the scarcity of reports, that the rocket contained no chemical payload but struck a chemical factory or warehouse.
Sarin suspected
However most experts agree that the videos of the people affected indicate something more sinister. "They provide individual visual evidence of people who are clearly affected by something," says Howard Hu of the University of Toronto, Canada, a medical advisor for the non-profit group Physicians for Human Rights.
Hu says the victims' rigid muscles and spasmodic movements suggest a neurological organophosphate toxin, such as sarin, but only good close-up images would allow for a more definitive assessment of the symptoms of sarin poisoning, which include drooling and constricted pupils.
Alastair Hay at the University of Leeds, UK, who has investigated a number of real and alleged chemical attacks, agrees. "Many of the victims have individual signs suggestive of exposure to an organophosphate agent. Pinpoint pupils are certainly one of the signs but [pupils] should not be used to rule out exposure: when people are afraid their pupils dilate and this may be the initial appearance".
The victims in the footage also show clear signs of asphyxiation, says Jean-Pascal Zanders, formerly of the European Institute of Security Studies in Paris. "With asphyxiation, you see colouring of the skin – a blue-pinkish hue, and a waxen look," he says. This is a sign the nervous system is affected and the major organs – for example, the heart and the lungs – are having trouble communicating with each other.
Victims hold clues
According to the US Congressional Research Service, the Syrian government is believed to have stockpiles of the nerve agents VX and sarin, as well as the blister agent, mustard gas.
If they can get to the scene, UN inspectors could gather more decisive evidence, but the Syrian government has so far been reluctant to grant full access to the site. The amount of time that any evidence will remain there for will depend on what the agent is. In a temperate climate, sarin evaporates at a similar rate to water, says Zanders, but the processes by which it breaks down, and the metabolites it produces in the body, are well known. This means it should be possible to detect even if the inspectors don't get in immediately. "Some things can be traced many months after the incident," Zanders says.
If the inspectors do gain access, the first order of business will be to collect physiological samples from the living victims and the bodies of dead ones. These could include tissue, blood, urine, hair and skin samples. If they were able, full post-mortems of the victims would be useful. The inspectors could also collect environmental samples, like leaves, soil or wallpaper and the corpses of any affected animals.
Covert agents
In the past, weapons inspectors have used portable equipment to run gas chromatography and mass spectrometry on the samples, which can separate the samples into their physical components and analyse them.
If this team doesn't have that equipment in the field, they would have to freeze the biological samples and ship them to a lab certified by the Organisation for the Prohibition of Chemical Weapons.
There's always the chance that samples will be smuggled out of Syria for analysis before the inspectors gain access. President Obama has instructed the US intelligence community to gather information about the recent attack. This may involve using covert agents to gather samples and smuggle them out of the country, as they have done in past conflicts.
Of course – the most conclusive evidence would be an analysis of the remains of the munition used to deliver the weapon. This could prove that it was a malicious attack, and what chemical was deployed.
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